In the medical field, new medicines, treatment methods and procedures are introduced at such a dazzling pace that even medical experts struggle to stay up to date. In the field of diagnostics many advances have also been made. If a disease can be diagnosed during its early stages it is easier to treat and the chances of success are much better. ELISA kits have proven to be a major breakthrough. The enzyme linked immunosorbent assay enables diagnosticians to recognize certain conditions quickly.
The EIA test, as it is also known, is used to analyze a sample of blood in order to determine the concentration of antibodies and antigens. It would be very difficult to make an accurate diagnosis if the concentration of the antigens and antibodies cannot be determined. Doctors favor this test because it is relatively easy to perform and the results are available quickly.
Antibodies are protein substances that are produced by the body when it detects the presence of dangerous substances. The dangerous substances are called antigens. When antibodies are detected by means of the EIA test, it follows logically that there must be antigens too. The exact identity of the antigens can be determined by identifying the type of antibody in the sample. In this way a diagnosis can be made confidently.
Patients experience no pain when the test is administered. A small quantity of blood is taken and then sent to a laboratory where a technician has already prepared a number of petri dishes, each containing a different antigen. The blood sample is then added to the dishes. If the antibodies in the blood samples react to any of the antigens they can be identified.
There are different forms of the EIA test, ranging from very simple to extremely complex. Different kits are used for the different forms of the test. With the direct EIA test, for example, the antigen is first covered with a multiwall plate. In this case the antibody in the blood sample will only react with the antigen if specific enzymes are added.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
Matched pairs of antibodies are used in the processing of the sandwich EIA test. In this case one of the antibodies is coated with polystyrene while the second one acts as a detecting agent. This test is performed when it is deemed necessary to determine the concentration of analytes in the sample. It is extremely accurate and can even detect diseases that have not manifested yet.
Any test or procedure that can help physicians to diagnose a disease at an earlier stage can help save lives. The EIA test certainly enables the accurate and early diagnosis of a variety of conditions. This can help ensure that treatment programs are less invasive and that the prognosis is better.
The EIA test, as it is also known, is used to analyze a sample of blood in order to determine the concentration of antibodies and antigens. It would be very difficult to make an accurate diagnosis if the concentration of the antigens and antibodies cannot be determined. Doctors favor this test because it is relatively easy to perform and the results are available quickly.
Antibodies are protein substances that are produced by the body when it detects the presence of dangerous substances. The dangerous substances are called antigens. When antibodies are detected by means of the EIA test, it follows logically that there must be antigens too. The exact identity of the antigens can be determined by identifying the type of antibody in the sample. In this way a diagnosis can be made confidently.
Patients experience no pain when the test is administered. A small quantity of blood is taken and then sent to a laboratory where a technician has already prepared a number of petri dishes, each containing a different antigen. The blood sample is then added to the dishes. If the antibodies in the blood samples react to any of the antigens they can be identified.
There are different forms of the EIA test, ranging from very simple to extremely complex. Different kits are used for the different forms of the test. With the direct EIA test, for example, the antigen is first covered with a multiwall plate. In this case the antibody in the blood sample will only react with the antigen if specific enzymes are added.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
Matched pairs of antibodies are used in the processing of the sandwich EIA test. In this case one of the antibodies is coated with polystyrene while the second one acts as a detecting agent. This test is performed when it is deemed necessary to determine the concentration of analytes in the sample. It is extremely accurate and can even detect diseases that have not manifested yet.
Any test or procedure that can help physicians to diagnose a disease at an earlier stage can help save lives. The EIA test certainly enables the accurate and early diagnosis of a variety of conditions. This can help ensure that treatment programs are less invasive and that the prognosis is better.
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